Released on = April 16, 2007, 2:46 pm

Press Release Author = AnaSpec Inc.

Industry = Biotech

Press Release Summary = Interleukin-32 (IL-32), whose expression is increased
following activation by IL-2, was initially identified as a transcript (NK4)
selectively expressed in lymphocytes and NK cells.

Press Release Body = April 16, 2007 - San Jose, CA

Interleukin Antibodies
Interleukin-32 (IL-32), whose expression is increased following activation by IL-2,
was initially identified as a transcript (NK4) selectively expressed in lymphocytes
and NK cells.1 It was later re-isolated from an IL-18-treated lung carcinoma cell
line and re-named IL-32.2 IL-32 does not share sequence homology with known
cytokine families and is highly expressed in immune tissues, existing in at least
four differentially spliced isoforms. Because treatment of human monocytic and
mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as
TNF-, IL-8 and MIP-2, and because it is induced in human peripheral
lymphocyte cells after mitogen stimulation and in epithelial cells by IFN-,
it has been suggested that IL-32 may play a role in autoimmune and inflammatory
diseases such as rheumatoid arthritis.3
Rabbit anti-IL-32 polyclonal antibody was raised against a 15 amino acid peptide
from near the C-terminus of human IL-32 (Genbank accession No. AAH09401).
Anti-IL-32 reacts with IL-32 at the molecular weight of approximately 22-25 kDa on
western blot. Anti-IL-32 detects all isoforms of IL-32.

Chemokine Antibodies
G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the
chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28
have been identified as HIV coreceptors.4 These coreceptors, as well as CD4, are
needed to infect target cells. CXCR4 (fusin, LESTR or HUMSTR) is a principal
coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood
cells.5,6 It is required for infection by the dual-tropic strains of HIV-1 and
mediates CD-4 independent infection by HIV-2.7,8 The  -chemokine SDF-1 is
the ligand for CXCR4 and prevents infection by T-tropic HIV-1.9,10 CXCR4 associates
with the surface CD4-gp120 complex before HIV enters target cells.11 CXCR4 messenger
RNA levels correlated with HIV-1 permissiveness in diverse human cell types.2
Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target
cells.5,8,13 The amino-terminal domain and the second extracellular loop of CXCR4
serve as HIV binding sites.13,14 Rabbit polyclonal CXCR4 antibody was raised
against a peptide corresponding to amino acids near the amino terminus of human
CXCR4.6,12

AnaSpec, a worldwide provider of custom and catalog antibodies, has added hundreds
of antibodies to its existing portfolio, including a number of interleukin &
chemokine antibodies.

For more information visit www.anaspec.com

AnaSpec, Inc. is a leading provider of integrated proteomics solutions to
pharmaceutical, biotech, and academic research institutions throughout the world.
With a vision for innovation through synergy, AnaSpec focuses on three core
technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and
combinatorial chemistry. Established in 1993, AnaSpec\'s ISO9001:2000 certified
headquarters and manufacturing facilities are located in San Jose, CA.

REFERENCES:
1. Dahl, CA. et al. J. Immunol. 148, 597 (1992).
2. Kim, SH. et al. Immunity 22, 131 (2005).
3. Cagnard, N. et al. Eur. Cyto. Network 16, 289 (2005).
4. Dimitrov DS. Cell 91, 721 (1997).
5. Feng, Y. et al. Science 272, 872 (1996).
6. Berson, JF. et al. J. Virol. 70, 6288 (1996).
7. Doranz, BJ. et al. Cell 85, 1149 (1996).
8. Endres, MJ. et al. Cell 87, 745 (1996).
9. Bleul, CC. et al. Nature 382, 829 (1996).
10. Oberlin, E. et al. Nature 382, 833 (1996).
11. Lapham, CK. et al. Science 274, 602 (1996).
12. Leoetscher, M. et al. J. Biol. Chem. 269, 232 (1994).
13. Brelot, A. et al. J. Virol. 71, 4744 (1997).
14. Lu, Z. et al. Proc. Natl. Acad. Sci. USA 94, 6426 (1997).




Web Site = http://www.anaspec.com

Contact Details = 2149 O\'toole Ave.
San Jose, CA 95131
1-408-452-5055
ping@anaspec.com